16.10 - 18.00 | Session 3: Live Imaging of cell, tissue and organisms
Chairs: Paul Timpson & Paul Macmillan
16.10 - 17.00 | Technique in Focus
- In situ visualisation of the plasma cell niche in blood cancer and autoimmunity.
Associate Professor Edwin Hawkins, Walter and Eliza Hall Institute - Intravital imaging technology guides FAK mediated priming in pancreatic cancer precision medicine according to Merlin status
Ms Kendall Murphy, Garvan Medical Research Institute - Intravital subcellular and single molecule imaging reveals multiple actin filament populations collaborate in the remodelling of the secretory granule membrane
Mr Marco Heydecker, UNSW Sydney
(1 x 20min + 2 x 10min + 10 min Q&A)
17.00 - 18.00 | Plenary
Recordings
Q&A Session
Technique in Focus Q&A
Keynote Presentation Q&A
Chat Transcript
00:11:55 Paul Mcmillan: Remember to post any questions you have in the chat and we will answer them at the end of the session.
00:19:11 Renee Whan: HI Ed, How many different combinations are possible with your confetti model?
00:32:48 Paul Timpson: Ed have you looked at photoswitching in your models
00:33:12 Paul Timpson: As cell move or progress coupled with RNAseq
00:37:39 Sarah Ellis: @Edwin. Lovely presentation (as usual 🙂). You mentioned that the clonality was more prevalent in long bones compared to the calvaria. Was this in relation to clone size and/or clone numbers? Is it simply a result of the increased volume of bone marrow in long bones?
00:42:27 Samantha Stehbens: @kendall – great talk; * are there any clinical trials running investigating FAKi in solid cancers with Gem/abraxane? * Which FAKi did you use in your study? *Did you look at FAK localisation in your models?
00:48:02 Emma Gordon: @Kendall, fabulous talk. I may have missed it in all the detail, but did you say that FAK activity is induced by stiffness? If so, any idea how that might occur?
00:52:39 Renee Whan: you can do it
00:57:31 Samantha Stehbens: thank-you! Just wondering about nuclear FAK
01:01:34 Paul Timpson: Remember to post any questions you have in the chat and we will answer them at the end of the talk.
01:32:52 Jonathan Teo: @Leonard, Thank you for such a fascinating talk. This might be a naïve question but are the animals with their heads fixed able to rear (stand on their hind limbs)?
01:33:17 Renee Whan: Astonishing….
01:33:32 Thomas Cox: Amazing talk! Could you combine this with optogenetic approaches to simultaneously neuromodulate and monitor response?
01:34:38 James Fraser: To follow on from that, what about axial rotation of the head?
01:34:42 Renee Whan: Have you examined whether you can use in vigilo, for immune models?
01:36:04 Edwin Hawkins: Amazing work. Do you make a Paul Timpson sized version of this system?
01:39:56 Paul Timpson: i do not want anyone to know what goes on in my head..ed
In situ visualisation of the plasma cell niche in blood cancer and autoimmunity.
Associate Professor Edwin Hawkins, Walter and Eliza Hall Institute
The generation of antibody that protects an individual for their lifetime is the cornerstone of the immune system. However, the mechanisms that select for, and maintain long-lived antibody producing cells in the bone marrow are not completely understood. By combining novel lineage tracing mouse models, multi-day 4 dimensional whole organ intravital imaging and single cell genomics, we have characterised a specialised niche in the bone marrow that drives this process. We illustrate how these niches lead to clonal selection of pathogenic plasma cells that drive diseases such as Lupus (autoimmunity) and Myeloma (an incurable blood cancer).
Synergistic Combination of Reductionist and Holistic Approaches in the in vigilo Experiments on Awake Behaving Mice".
Dr Leonard Khirug, University of Helsinki
After decades of reductionist research to Neuroscience, animal experimentation is becoming increasingly more holistic as multimodal imaging and recording techniques are combined in a single experiment on the brain of awake behaving animal. This newly emerged approach can be called in vigilo (from Latin 'in awake' or 'in vigilant state'). The reductionist phase was the time to cast away stones as we kept splitting the subject matter into smaller and smaller “building blocks”. In this talk, I will argue that it is now time to gather stones, and indeed this has been a dominant trend during the last two decades.
Intravital subcellular and single molecule imaging reveals multiple actin filament populations collaborate in the remodelling of the secretory granule membrane
Mr Marco Heydecker, UNSW Sydney
Intravital imaging technology guides FAK mediated priming in pancreatic cancer precision medicine according to Merlin status
Ms Kendall Murphy, Garvan Medical Research Institute